DetScan^™

Invented by scientists from the Laboratoire d’Électrochimie Moléculaire (LEM-Université Paris Diderot), Damien Marchal and Benoit Limoges, DetScan™ is protected by several patents. Based on electrochemistry, it allows a non-optical and parallelised detection of biological molecules (DNA, RNA and proteins).

As many approaches in the biosensor field, DetScan™ technology has the particularity to gather all the advantages of the electronic industry in terms of robustness, cost and miniaturization potential.

Principle of DetScan™

DetScan™ technology is based on a measurement of the interaction between an electrochemical binding probe and a biological molecule.

Technology Principle

Applications

DetScan™ could be considered as a generic technology which allows the development of several applications in detection, analysis and quantification of a biological molecule in a sample.

DetScan™ technology was for the first time described in 2006 for a non-optical real-time PCR experiment using cyclic voltammetry. Since this proof of principle, it has been optimised and adapted to several and daily used applications in molecular biology.

Isothermal amplification experiment

Electrochemical Real-Time PCR experiments

Electrochemical melt curves

Genotyping experiment

Expand beyond boundaries

• Electrochemistry used for biological molecules detection is an open gate to new applications.
• Robustness, miniaturization and low cost procedures are key criteria favoured by new players such as the Diagnostic and the Agro-food industries.
• In the PCR field, DetScan™ and Leo will lure new users to PCR.

References

Real-Time Electrochemical Monitoring of Isothermal Helicase-Dependent Amplification of Nucleic Acids, F. Kivlehan, F. Mavré, L. Talini, B. Limoges, D. Marchal. Analyst, 2011, 136, 3635-3642

Real-Time Electrochemical PCR with a DNA Intercalating Redox Probe, T. Deféver, M. Druet, D. Evrard, D. Marchal, B. Limoges. Anal. Chem., 2011 83 (5), 1815-1821

Real-Time Electrochemical Monitoring of the Polymerase Chain Reaction by Mediated Redox Catalysis, T. Deféver, M. Druet, M. Rochelet-Dequaire, M. Joannes, C. Grossiord, B. Limoges, D. Marchal. J. Am. Chem. Soc., 2009, 131 (32), 11433–11441

Detection of a few DNA copies by real-time electrochemical polymerase chain reaction. M. Moreau, S. Delile, A. Sharma, C. Fave, A. Perrier, B. Limoges, D. Marchal. Analyst, 2017, 142, 18, 3432-3440.

Ultimate single-copy DNA detection using real-time electrochemical LAMP, A. Martin, K. Grant, F. Stressmann, Franziska, J-M. Ghigo, D. Marchal, B. Limoges, ACS sensors. 2016, 1, 904-912

Real-time electrochemical LAMP: a rational comparative study of different DNA intercalating and non-intercalating redox probes, A. Martin, L. Bouffier, K. Grant, B. Limoges, D. Marchal, Analyst, 2016, 141, 4196-4203

Simple Homogeneous electrochemical monitoring of exonuclease III activity and its application to nucleic acid testing by target recycling, R. Miranda-Castro, D. Marchal, B. Limoges, F. Mavré. Chem. Com. 2012, 48(70), 8772-8774 

Real-Time Electrochemical Monitoring of Isothermal Helicase-Dependent Amplification of Nucleic Acids, F. Kivlehan, F. Mavré, L. Talini, B. Limoges, D. Marchal, Analyst, 2011, 136, 3635-3642.

Real-Time Electrochemical PCR with a DNA Intercalating Redox Probe, T. Deféver, M. Druet, D. Evrard, D. Marchal, B. Limoges, Anal. Chem., 2011 83 (5), 1815-1821.

Real-Time Electrochemical Monitoring of the Polymerase Chain Reaction by Mediated Redox Catalysis, T. Defever, M. Druet, M. Rochelet-Dequaire, M. Joannes, C. Grossiord, B. Limoges,  D. Marchal. J. Am. Chem. Soc., 2009, 131 (32), 11433–11441